CCK-8 Assay:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
DNA Ligation:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
Protease Inhibitor:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
Purification:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
RNA Extraction:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
Reverse Transcription:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
SYBR Green Assay:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
Real-time Polymerase Chain Reaction:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
Labeling:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
Staining:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
Enzyme-linked Immunosorbent Assay:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
|
MedChemExpress is a verified supplier 
MedChemExpress manufactures this product 